Combined single-molecule force and fluorescence measurements for biology
National Institute for Medical Research, London NW7 1AA, UK
Journal of Biology 2003, 2:4 doi:10.1186/1475-4924-2-4Published: 14 April 2003
Recent advances in single-molecule techniques allow the application of force to an individual biomolecule whilst simultaneously monitoring its response using fluorescent probes. The effects of applied mechanical load on single-enzyme turnovers, biomolecular interactions and conformational changes can now be studied with nanometer precision and millisecond time resolution.