|
Overview of identified molecular targets |
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| Gene |
Domain homology |
Direction |
Accession number* |
Relative to ORF* |
Frequency* |
Phenotype transfer |
|
|
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| Known to function in TCR pathway |
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| TCRβ |
Receptor |
Sense |
Numerous |
Partial |
46 |
On hold |
| ZAP70 |
Tyrosine kinase |
Sense |
L05148.1 |
-147, +787 nt |
12 |
Yes |
| ZAP70 (long) |
Tyrosine kinase |
Sense |
L05148.1 |
-21, +809 nt |
17 |
TBD |
| Syk |
Tyrosine kinase |
Sense |
L28824.1 |
-27, +1012 nt |
2 |
Yes |
| Lck |
Tyrosine kinase |
Sense |
U23852.1 |
-59, +799 nt |
4 |
Yes |
| PLCγ1 |
Tyrosine kinase |
Sense |
NM_002660.1 |
+1409, +2282 nt |
3 |
Yes |
| SHP-1/PTP1C |
Protein-tyrosine phosphatase |
Sense |
X62055.1 |
+472, >+2021 nt |
1 |
Yes |
| Csk |
Tyrosine kinase |
Sense |
NM_004383.1 |
-55, +1285 nt |
1 |
TBD |
| PAG |
Transmembrane adaptor |
Sense |
NM_018440.2 |
-237, +644 nt |
1 |
Yes |
| Nucleolin |
RNA-binding |
Sense |
NM_005381.1 |
-136, +479 nt |
1 |
No |
| Enzymes and receptors |
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| TCPTP/PTPN2 |
Protein-tyrosine phosphatase |
Sense |
NM_002828.1 |
-58, +1108 nt |
20 |
Yes |
| PAK2 |
p21-activated kinase 2 |
Sense |
NM_002577.1 |
-50, +339 nt |
18 |
Yes |
| PAK2 (long) |
p21-activated kinase 2 |
Sense |
NM_002577.1 |
-42, +670 nt |
1 |
Yes |
| A-Raf-1 |
Serine/threonine kinase |
Sense |
X04790.1 |
-4, +456 nt |
5 |
Yes |
| EDG1 |
G-protein-coupled receptor |
Sense |
NM_001400.2 |
<-244, +942 nt |
4 |
Yes |
| EDG1 (long) |
G-protein-coupled receptor |
Sense |
NM_001400.2 |
<-244, +1037 nt |
1 |
TBD |
| TRAC-1 |
RING finger ubiquitin ligase |
Sense |
NM_017831.1 |
-254, +510 nt |
1 |
Yes |
| IL-10Rα |
Receptor |
Sense |
NM_001558.1 |
+689, +1350 nt |
1 |
Yes |
| Integrin α2 |
Receptor |
Sense |
NM_002203.2 |
+3348, +3914 nt |
1 |
Yes |
| Enolase 1α |
Phosphopyruvate hydratase |
Sense |
NM_001428.1 |
+703, +1374 nt |
2 |
No |
| DUSP1 |
Dual-specificity phosphatase |
Sense |
NM_004417.2 |
+817, +1112 nt |
1 |
No |
| KIAA0251 |
Pyridoxal-dependent decarboxylase |
Sense |
D87438.1 |
nt 2098-2370† |
1 |
No |
| Adaptors and transcription factors |
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| Grb7 |
Adaptor |
Sense |
NM_005310.1 |
+1268, +1912 nt |
3 |
Yes |
| GG2-1 |
TNF-induced protein |
Sense |
AF070671.1 |
-97, +1795 nt |
2 |
Yes |
| SH2-B |
Adaptor |
Sense |
AF227968.1 |
+1352, +1960 nt |
1 |
Yes |
| RERE |
Transcriptional factor |
Sense |
AB036737.1 |
+914, +1202 nt |
3 |
No |
| SudD |
Serine/threonine rich |
Sense |
NM_003831.1 |
-93, +413 nt |
1 |
No |
| Ku 70 |
DNA-PKc subunit |
Sense |
S38729.1 |
+1026, +2069 nt |
1 |
No |
| Novel (130 amino acids; no homology) |
Unknown |
Sense |
AC005321.1 |
nt 33543-33938‡ |
1 |
No |
| Novel signaling molecule |
GYF domain |
Sense |
NM_022574.1 |
+1, +121 nt |
1 |
No |
| SCAMP2 |
Secretory carrier membrane protein |
Sense |
AF005038.2 |
-5, +833 nt |
1 |
No |
| KIAA1228 |
C2 domain (Ca2+- or IP-binding) |
Sense |
AB033054.2 |
nt 1439-2163† |
1 |
No |
| EST from clone 2108068 |
LPP20 lipoprotein precursor |
Sense |
Al357532.1 |
Novel isoform |
1 |
No |
| RNH |
Ribonuclease/angiogenin inhibitor |
Sense |
NM_002939.1 |
-22, +713 nt |
1 |
No |
| Cytoskeleton |
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| Moesin |
Moesin |
Sense |
NM_002444.1 |
-93, +1534 nt |
1 |
Yes |
| Vimentin |
Intermediate filament |
Sense |
NM_003380.1 |
-98, +374 nt |
1 |
Yes |
| Others |
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| Alu repeat |
5 |
On hold |
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| CpG island? (clone 550H1) |
AL035420.1 |
1 |
On hold |
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| IgG2 heavy chain |
Ig superfamily |
Sense |
Partial |
1 |
On hold |
|
| Ig light chain |
Ig superfamily |
Sense |
Partial |
1 |
On hold |
|
| 18S rRNA |
Sense |
M10098.1 |
Partial |
2 |
On hold |
|
| 28S rRNA |
Sense |
Partial |
1 |
On hold |
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*For each identified clone, the GenBank database [51] accession number is given, followed by the first and last nucleotide (nt) positions relative to the initiation codon (ATG being the +1, +2, +3 nts, respectively); Frequency indicates the number of original cell clones expressing the specific hit. †Relative to the EST itself because the start codon is not identified. ‡Relative to the genomic clone itself. Ig, Immunoglobulin; TBD, to be determined. | ||||||
Chu et al. Journal of Biology 2003 2:21 doi:10.1186/1475-4924-2-21 |
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