Table 1

Overview of identified molecular targets

Gene

Domain homology

Direction

Accession number*

Relative to ORF*

Frequency*

Phenotype transfer


Known to function in TCR pathway

TCRβ

Receptor

Sense

Numerous

Partial

46

On hold

ZAP70

Tyrosine kinase

Sense

L05148.1

-147, +787 nt

12

Yes

ZAP70 (long)

Tyrosine kinase

Sense

L05148.1

-21, +809 nt

17

TBD

Syk

Tyrosine kinase

Sense

L28824.1

-27, +1012 nt

2

Yes

Lck

Tyrosine kinase

Sense

U23852.1

-59, +799 nt

4

Yes

PLCγ1

Tyrosine kinase

Sense

NM_002660.1

+1409, +2282 nt

3

Yes

SHP-1/PTP1C

Protein-tyrosine phosphatase

Sense

X62055.1

+472, >+2021 nt

1

Yes

Csk

Tyrosine kinase

Sense

NM_004383.1

-55, +1285 nt

1

TBD

PAG

Transmembrane adaptor

Sense

NM_018440.2

-237, +644 nt

1

Yes

Nucleolin

RNA-binding

Sense

NM_005381.1

-136, +479 nt

1

No

Enzymes and receptors

TCPTP/PTPN2

Protein-tyrosine phosphatase

Sense

NM_002828.1

-58, +1108 nt

20

Yes

PAK2

p21-activated kinase 2

Sense

NM_002577.1

-50, +339 nt

18

Yes

PAK2 (long)

p21-activated kinase 2

Sense

NM_002577.1

-42, +670 nt

1

Yes

A-Raf-1

Serine/threonine kinase

Sense

X04790.1

-4, +456 nt

5

Yes

EDG1

G-protein-coupled receptor

Sense

NM_001400.2

<-244, +942 nt

4

Yes

EDG1 (long)

G-protein-coupled receptor

Sense

NM_001400.2

<-244, +1037 nt

1

TBD

TRAC-1

RING finger ubiquitin ligase

Sense

NM_017831.1

-254, +510 nt

1

Yes

IL-10Rα

Receptor

Sense

NM_001558.1

+689, +1350 nt

1

Yes

Integrin α2

Receptor

Sense

NM_002203.2

+3348, +3914 nt

1

Yes

Enolase 1α

Phosphopyruvate hydratase

Sense

NM_001428.1

+703, +1374 nt

2

No

DUSP1

Dual-specificity phosphatase

Sense

NM_004417.2

+817, +1112 nt

1

No

KIAA0251

Pyridoxal-dependent decarboxylase

Sense

D87438.1

nt 2098-2370

1

No

Adaptors and transcription factors

Grb7

Adaptor

Sense

NM_005310.1

+1268, +1912 nt

3

Yes

GG2-1

TNF-induced protein

Sense

AF070671.1

-97, +1795 nt

2

Yes

SH2-B

Adaptor

Sense

AF227968.1

+1352, +1960 nt

1

Yes

RERE

Transcriptional factor

Sense

AB036737.1

+914, +1202 nt

3

No

SudD

Serine/threonine rich

Sense

NM_003831.1

-93, +413 nt

1

No

Ku 70

DNA-PKc subunit

Sense

S38729.1

+1026, +2069 nt

1

No

Novel (130 amino acids; no homology)

Unknown

Sense

AC005321.1

nt 33543-33938

1

No

Novel signaling molecule

GYF domain

Sense

NM_022574.1

+1, +121 nt

1

No

SCAMP2

Secretory carrier membrane protein

Sense

AF005038.2

-5, +833 nt

1

No

KIAA1228

C2 domain (Ca2+- or IP-binding)

Sense

AB033054.2

nt 1439-2163

1

No

EST from clone 2108068

LPP20 lipoprotein precursor

Sense

Al357532.1

Novel isoform

1

No

RNH

Ribonuclease/angiogenin inhibitor

Sense

NM_002939.1

-22, +713 nt

1

No

Cytoskeleton

Moesin

Moesin

Sense

NM_002444.1

-93, +1534 nt

1

Yes

Vimentin

Intermediate filament

Sense

NM_003380.1

-98, +374 nt

1

Yes

Others

Alu repeat

5

On hold

CpG island? (clone 550H1)

AL035420.1

1

On hold

IgG2 heavy chain

Ig superfamily

Sense

Partial

1

On hold

Ig light chain

Ig superfamily

Sense

Partial

1

On hold

18S rRNA

Sense

M10098.1

Partial

2

On hold

28S rRNA

Sense

Partial

1

On hold


*For each identified clone, the GenBank database [51] accession number is given, followed by the first and last nucleotide (nt) positions relative to the initiation codon (ATG being the +1, +2, +3 nts, respectively); Frequency indicates the number of original cell clones expressing the specific hit. Relative to the EST itself because the start codon is not identified. Relative to the genomic clone itself. Ig, Immunoglobulin; TBD, to be determined.

Chu et al. Journal of Biology 2003 2:21   doi:10.1186/1475-4924-2-21

Open Data