Table 1 |
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|
RNAi screen results classified by predicted gene function |
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| Genes identified* |
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|
|
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| Gene class† |
N |
Total |
Total S2R+ |
Total Kc167 |
Both‡ |
||||
|
|
|||||||||
| Kinase |
229 |
54 |
18.8% |
3 |
18.8% |
2 |
12.5% |
2 |
12.5% |
| Miscellaneous |
139 |
16 |
11.5% |
15 |
10.8% |
11 |
7.9% |
10 |
7.2% |
| Cytoskeletal |
116 |
17 |
14.7% |
17 |
14.7% |
11 |
9.5% |
11 |
9.5% |
| Motor |
77 |
7 |
9.1% |
6 |
7.8% |
3 |
3.9% |
2 |
2.6% |
| Phosphatase |
72 |
12 |
16.7% |
10 |
13.9% |
7 |
9.7% |
5 |
6.9% |
| GTPase |
54 |
15 |
27.8% |
15 |
27.8% |
6 |
11.1% |
6 |
11.1% |
| Transport |
48 |
2 |
4.2% |
1 |
2.1% |
2 |
4.2% |
1 |
2.1% |
| Proteolysis |
42 |
7 |
16.7% |
7 |
16.7% |
6 |
14.3% |
6 |
14.3% |
| Lipid-associated |
38 |
3 |
7.9% |
3 |
7.9% |
0 |
0% |
0 |
0% |
| GEF |
32 |
8 |
25.0% |
7 |
21.9% |
3 |
9.4% |
2 |
6.3% |
| PDZ |
32 |
3 |
9.4% |
3 |
9.4% |
1 |
3.1% |
1 |
3.1% |
| GAP |
31 |
6 |
19.4% |
5 |
16.1% |
2 |
6.5% |
1 |
3.2% |
| SH2/SH3 |
25 |
3 |
12.0% |
2 |
8.0% |
1 |
4.0% |
0 |
0% |
| Adhesion |
23 |
3 |
13.0% |
3 |
13.0% |
0 |
0% |
0 |
0% |
| Cyclase |
20 |
1 |
5.0% |
0 |
0% |
1 |
9.5% |
0 |
0% |
| G protein |
16 |
3 |
18.8% |
3 |
18.8% |
2 |
12.5% |
2 |
12.5% |
| Total genes |
994 |
160 |
16.1% |
146 |
14.7% |
79 |
7.9% |
65 |
6.5% |
|
In total, we screened 1,061 wells, 1,042 dsRNAs, 994 genes and found 160 genes with phenotypes. *The number and percentage of genes identified with any RNAi phenotype in duplicate screens. †The total number of genes (N) represented in the dsRNA set as defined by amino-acid sequence and Gene Ontology [33] or FlyBase [12] annotation. Each gene was counted in only one category. ‡Genes identified by phenotypes in both Kc167 and S2R+ cells. | |||||||||
Kiger et al. Journal of Biology 2003 2:27 doi:10.1186/1475-4924-2-27 |
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