Table 1

Screens for lamellar and cytokinesis phenotypes in cultured Drosophila cells

Phenotype:

Cytokinesis

Lamellar

Actin/shape


Cell type:

S2

S2

S2R, Kc167

S2 cells

S2R, Kc167

Study:

Somma et al. [9]

Rogers et al. [10]; Goshima and Vale [7]

Kiger et al. [8]

Rogers et al. [10]

Kiger et al. [8]

Protein (gene)*


Myosin-II (zipper)

NT

+

-

-

-

Myosin RLC (spaghetti squash)

+

NT

+

NT

-

Profilin (chickadee)

-

+

-

+

+

CAP (act up/capulet)

NT

+

-

+

+

Cofilin (twinstar)

+

+

+

+

+

Actin interacting protein 1; Aip1

NT

+

NT

+

NT

Formin (diaphanous)

NT

+

+

-

+

Anillin (scraps)

+

+

+

-

+

Kinesin (pavarotti)

+

+

+

-

-

Rho1 GTPase

+

+

+

+

-

Rho GEF (pebble)

+

NT

+

NT

-

RacGAP; RacGAP50C

+

+

-

-

-

Syntaxin1A

+

NT

NT

NT

NT

Dynamin (shibire)

NT

NT

+

NT

-

Citron kinase

NT

+

+

-

-

Aurora kinase

NT

NT

+

NT

+

Arp2/3 complex

NT

-

-

+

+

Scar

NT

-

-

+

+

Cortactin

NT

-

-

-

+

Nck (dock)

NT

-

-

+

-

Cdc42 GTPase

NT

-

-

+

+

Rac1/2 GTPase

NT

-

-

-

+ (Rac2 only)

Capping protein

NT

-

-

+

+

Abl kinase

NT

-

-

+

+

Abelson-interacting protein (abi)

NT

-

-

+

-

Enabled kinase

NT

-

-

-

+

Rho kinase

NT

NT

-

NT

+

PAK

NT

- (PAK1)

-

-

+ (PAK3)

Cofilin phosphatase (slingshot)

NT

-

-

+

+

Rho GEF (vav)

NT

-

-

-

+

PIP3 phosphatase (pten)

NT

NT

-

NT

+

Other myosins

NT

-

-

-

+ (uncharacterized ORF, CG15780)

Polo kinase

NT

NT

+

NT

+

LIM kinase

NT

-

-

-

-

Ciboulot

NT

-

-

-

-

Coronin

NT

-

NT

-

NT

Filamin

NT

-

- (jitterbug)

-

- (jitterbug)

Gelsolin

NT

-

-

-

-

Tropomodulin (sanpodo)

NT

-

-

-

-

Tropomyosin

NT

-

NT

-

NT

Septin (peanut)

-

NT

NT

NT

NT

WASP

NT

-

-

-

-


+ indicates that a cytokinesis or lamellar phenotype was observed; - no phenotype was observed; NT, not tested. *Protein names (sometimes more than one alternative) are given in roman type and gene names in italics. In unpublished experiments, accumulation of actin and disruption of the cell monolayer was found with profilin RNAi and a cytokinesis phenotype was seen with zipper RNAi (A. Kiger, personal communication). Mitotic phenotypes were observed.

Pollard Journal of Biology 2003 2:25   doi:10.1186/1475-4924-2-25