|
Screens for lamellar and cytokinesis phenotypes in cultured Drosophila cells |
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| Phenotype: |
Cytokinesis |
Lamellar |
Actin/shape |
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|
|
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| Cell type: |
S2 |
S2 |
S2R, Kc167 |
S2 cells |
S2R, Kc167 |
| Study: |
Somma et al. [9] |
Rogers et al. [10]; Goshima and Vale [7] |
Kiger et al. [8] |
Rogers et al. [10] |
Kiger et al. [8] |
| Protein (gene)* |
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|
|
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| Myosin-II (zipper) |
NT |
+ |
-† |
- |
-† |
| Myosin RLC (spaghetti squash) |
+ |
NT |
+ |
NT |
- |
| Profilin (chickadee) |
- |
+ |
-† |
+ |
+† |
| CAP (act up/capulet) |
NT |
+ |
- |
+ |
+ |
| Cofilin (twinstar) |
+ |
+ |
+ |
+ |
+ |
| Actin interacting protein 1; Aip1 |
NT |
+ |
NT |
+ |
NT |
| Formin (diaphanous) |
NT |
+ |
+ |
- |
+ |
| Anillin (scraps) |
+ |
+ |
+ |
- |
+ |
| Kinesin (pavarotti) |
+ |
+ |
+ |
- |
- |
| Rho1 GTPase |
+ |
+ |
+ |
+ |
- |
| Rho GEF (pebble) |
+ |
NT |
+ |
NT |
- |
| RacGAP; RacGAP50C |
+ |
+ |
- |
- |
- |
| Syntaxin1A |
+ |
NT |
NT |
NT |
NT |
| Dynamin (shibire) |
NT |
NT |
+ |
NT |
- |
| Citron kinase |
NT |
+ |
+ |
- |
- |
| Aurora kinase |
NT |
NT |
+ |
NT |
+ |
| Arp2/3 complex |
NT |
- |
- |
+ |
+ |
| Scar |
NT |
- |
- |
+ |
+ |
| Cortactin |
NT |
- |
- |
- |
+ |
| Nck (dock) |
NT |
- |
- |
+ |
- |
| Cdc42 GTPase |
NT |
- |
- |
+ |
+ |
| Rac1/2 GTPase |
NT |
- |
- |
- |
+ (Rac2 only) |
| Capping protein |
NT |
- |
- |
+ |
+ |
| Abl kinase |
NT |
- |
- |
+ |
+ |
| Abelson-interacting protein (abi) |
NT |
- |
- |
+ |
- |
| Enabled kinase |
NT |
- |
- |
- |
+ |
| Rho kinase |
NT |
NT |
- |
NT |
+ |
| PAK |
NT |
- (PAK1) |
- |
- |
+ (PAK3) |
| Cofilin phosphatase (slingshot) |
NT |
- |
- |
+ |
+ |
| Rho GEF (vav) |
NT |
- |
- |
- |
+ |
| PIP3 phosphatase (pten) |
NT |
NT |
- |
NT |
+ |
| Other myosins |
NT |
- |
- |
- |
+ (uncharacterized ORF, CG15780) |
| Polo kinase |
NT |
NT |
+‡ |
NT |
+‡ |
| LIM kinase |
NT |
- |
- |
- |
- |
| Ciboulot |
NT |
- |
- |
- |
- |
| Coronin |
NT |
- |
NT |
- |
NT |
| Filamin |
NT |
- |
- (jitterbug) |
- |
- (jitterbug) |
| Gelsolin |
NT |
- |
- |
- |
- |
| Tropomodulin (sanpodo) |
NT |
- |
- |
- |
- |
| Tropomyosin |
NT |
- |
NT |
- |
NT |
| Septin (peanut) |
- |
NT |
NT |
NT |
NT |
| WASP |
NT |
- |
- |
- |
- |
|
+ indicates that a cytokinesis or lamellar phenotype was observed; - no phenotype was observed; NT, not tested. *Protein names (sometimes more than one alternative) are given in roman type and gene names in italics. †In unpublished experiments, accumulation of actin and disruption of the cell monolayer was found with profilin RNAi and a cytokinesis phenotype was seen with zipper RNAi (A. Kiger, personal communication). ‡Mitotic phenotypes were observed. | |||||
Pollard Journal of Biology 2003 2:25 doi:10.1186/1475-4924-2-25 |
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