Levels of gene expression do not necessarily correlate with gene function. Immunoblot detection of anti-βPS-integrin (Mys, top panels) and anti-Enabled (Ena, middle panels) after 3 days RNAi. Columns represent Kc167 cells (left) and S2R+ cells (right) treated with different dsRNAs (gfp, ena, mys, R, talin). Both cell types expressed Mys and Ena in cells treated with a nonspecific dsRNA. The respective proteins were completely and specifically depleted by treatment with mys or ena dsRNAs. Anti-α-tubulin (bottom panels; Tub) shows a loading comparison.
Kiger et al. Journal of Biology 2003 2:27 doi:10.1186/1475-4924-2-27