Table 1

RNAi screen results classified by predicted gene function

Genes identified*


Gene class

N

Total

Total S2R+

Total Kc167

Both


Kinase

229

54

18.8%

3

18.8%

2

12.5%

2

12.5%

Miscellaneous

139

16

11.5%

15

10.8%

11

7.9%

10

7.2%

Cytoskeletal

116

17

14.7%

17

14.7%

11

9.5%

11

9.5%

Motor

77

7

9.1%

6

7.8%

3

3.9%

2

2.6%

Phosphatase

72

12

16.7%

10

13.9%

7

9.7%

5

6.9%

GTPase

54

15

27.8%

15

27.8%

6

11.1%

6

11.1%

Transport

48

2

4.2%

1

2.1%

2

4.2%

1

2.1%

Proteolysis

42

7

16.7%

7

16.7%

6

14.3%

6

14.3%

Lipid-associated

38

3

7.9%

3

7.9%

0

0%

0

0%

GEF

32

8

25.0%

7

21.9%

3

9.4%

2

6.3%

PDZ

32

3

9.4%

3

9.4%

1

3.1%

1

3.1%

GAP

31

6

19.4%

5

16.1%

2

6.5%

1

3.2%

SH2/SH3

25

3

12.0%

2

8.0%

1

4.0%

0

0%

Adhesion

23

3

13.0%

3

13.0%

0

0%

0

0%

Cyclase

20

1

5.0%

0

0%

1

9.5%

0

0%

G protein

16

3

18.8%

3

18.8%

2

12.5%

2

12.5%

Total genes

994

160

16.1%

146

14.7%

79

7.9%

65

6.5%


In total, we screened 1,061 wells, 1,042 dsRNAs, 994 genes and found 160 genes with phenotypes. *The number and percentage of genes identified with any RNAi phenotype in duplicate screens. The total number of genes (N) represented in the dsRNA set as defined by amino-acid sequence and Gene Ontology [33] or FlyBase [12] annotation. Each gene was counted in only one category. Genes identified by phenotypes in both Kc167 and S2R+ cells.

Kiger et al. Journal of Biology 2003 2:27   doi:10.1186/1475-4924-2-27

Open Data