Figure 2.

Endophilin 2 binds to Mo-MuLV Gag in vitro. (a) Bacterial lysates expressing either GST, GST-N125 or GST-ΔSH3 fragments of endophilin or GST-endophilin 2 (full-length; 'enph 2' on this and subsequent figures) were resolved by electrophoresis on 12% SDS gels, and the gel was stained with Coomassie Brilliant Blue. (b) Bacterial lysates expressing GST fusions were mixed with mammalian cell lysates either from Mo-MuLV chronically infected NIH 3T3 or from naïve NIH 3T3 cells. Proteins in the cell lysates that bound to GST fusion proteins were recovered with glutathione-Sepharose beads. Beads were then boiled in SDS sample buffer and the proteins were resolved by electrophoresis on 12% gels and analyzed by western blotting with an anti-capsid antibody.