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Interactions between endophilin 2 and retroviral Gag proteins in the yeast two-hybrid system |
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| pGADNOT-AD fusion |
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|
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| pSH2-LexA fusion |
Endophilin 2 |
Gag |
|
|
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| Mo-MuLV Pr65 Gag |
+++ |
++++ |
| RSV Pr65 Gag |
++/+ |
++++ |
| MPMV Pr76 Gag |
- |
++ |
| SIV Pr57 Gag |
- |
+++ |
| HIV-1 Pr55 Gag |
- |
++++ |
| Human endophilin 2 |
++++ |
|
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The various Gag proteins were fused to the DNA-binding domain, and human endophilin 2 was fused to the activation domain. All constructs tested negative for self-activation. Symbols: -, no blue color after 24 h in reaction; +, yeast turn blue after 2 h; ++, blue develops between 30 min and 2 h; +++, blue in approximately 30 min; ++++, blue between 15 and 30 min. | ||
Wang et al. Journal of Biology 2003 3:4 doi:10.1186/1475-4924-3-4 |
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