Nuclear export of Dsh is not critical for its activity. (a) The Dsh constructs used to analyze nuclear export. (b-d) RNAs encoding Dsh-GFP, Ds2 and DsNESm (0.5 ng each) were injected into two animal blastomeres of 4–8-cell embryos. Animal-cap explants were excised at stage 10, fixed and examined for GFP fluorescence. (b) Wild type Dsh-GFP localized in punctate structures of the cytoplasm, whereas (c) Ds2 and (d) DsNESm accumulated in the nucleus of animal pole cells. (e,f) One ventral vegetal blastomere of 8-cell embryos was injected with 1 ng Dsh-GFP or DsNESm RNA as indicated. Complete secondary axes were induced in both cases. (g) Uninjected sibling embryos.
Itoh et al. Journal of Biology 2005 4:3 doi:10.1186/jbiol20