Dsh mutants retain the ability to bind CK1ε and XARP. Four-cell embryos were injected in four sites in the animal hemisphere with CK1ε, HA-XARP, Myc-tagged Dsh, DsNLSm, Ds3 or DsSNLS RNA alone (2 ng each) or in combinations as indicated. The embryonic lysates were collected at stage 10.5 for immunoprecipitation with anti-Myc antibodies. Co-immunoprecipitated (a) CK1ε or (b) HA-XARP was probed with anti-CK1ε or anti-HA antibodies; β-tubulin served as a loading control.
Itoh et al. Journal of Biology 2005 4:3 doi:10.1186/jbiol20