Table 1

Axis induction by Dsh constructs

Injected RNA

Total number of injected embryos

Complete secondary axes (%)

Partial secondary axes (%)


Experiment 1

Dsh-GFP

150

46.6

25.3

DsNESm-GFP

194

54.6

30.4

Experiment 2

Dsh-GFP

144

28.5

45.1

DsNLSm-GFP

149

0.7

39.5

DsSNLS-GFP

137

24.0

42.3


Embryos were injected as described in Figure 1e,f. Partial secondary axes are defined by a morphologically visible ectopic neural tube up to the hindbrain level. Complete axes are defined by the presence of the secondary head structures, including eyes and cement glands. The frequency of secondary axes in uninjected embryos was less than 1%. Data pooled from several independent experiments are shown.

Itoh et al. Journal of Biology 2005 4:3   doi:10.1186/jbiol20

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