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Table 1
Dissociation constants for the binding of tRNA^fMetand tRNA^Pheto different ribosomal complexes
Set	State	Peptide	Codon in the E site	tRNA	Dissociation constant K_D(nM)	Additional factors; temperature

1	PreT	fMI	Thr (ACG)	fMet	144 ± 7	37°C
					179 ± 14	EF-G + GTP; 37°C
					770 ± 80	EF-G + GDP; 37°C
					40 ± 2	EF-G + GDPNP; 37°C
2	PostT	fMI	Met (AUG)	fMet	153 ± 9	37°C
				Phe	250 ± 30
		fM	Phe (UUU)	fMet	295 ± 26
				Phe	84 ± 11
		fMFTI	Thr (ACG)	fMet	162 ± 5
				Phe	134 ± 20
3	PostT	fMI	Met (AUG)	fMet	155 ± 10	+ RelE (A-site cut); 37°C
4	PostT	fMI	Met (AUG)	fMet	24.8 ± 1.2	0°C

Different conditions were used for measuring the dissociation constants for the different combinations of tRNA and ribosomal complexes as in Figure 7, and are shown in the last column.
Zavialov et al. Journal of Biology 2005 4:9 doi:10.1186/jbiol24

Table 1