Increased X-chromosome expression in the X;AA soma and germline. (a) Experimental design. The mRNA samples from XX;AA or X;AA somatic or germline tissues were labeled with either Cy3 or Cy5 and hybridized to FlyGEM arrays. Hybridization intensities corresponding to X chromosomes (red) and autosomal genes (black) were analyzed within each X:AA or XX:AA sample. (b-f) Histograms of hybridization intensities. (b) Autosomal hybridization intensities from single-copy (orange) and two-copy (black) regions of Df/+ samples in whole adult males, whole adult females and ovaries. Bars represent autosomal arms, in the order shown across the top. (c-f) Average hybridization intensities from the single X chromosome (red) compared to two copies of each autosomal arms. (c) Germlineless X;AA male progeny of homozygous tud1mothers and carcasses from X;AA males transformed into somatic females with hs-tra. (d) X;AA gonads from wildtype males and X;AA hs-tra sex transformed flies. (e) Germlineless XX;AA female progeny of homozygous tud1mothers and XX;AA females transformed into males by dsxswe/Df(3R)dsxM+15and tra2B/Df(2R)trix. (f) XX;AA gonads from wild-type and otu17/otu1and Sxl7BO/Sxlfs3transformed ovaries. Error bars show standard deviation of the mean hybridization intensity from all replicates of each sample. Numbers of hybridization replicates are indicated in parentheses above each histogram panel.
Gupta et al. Journal of Biology 2006 5:3 doi:10.1186/jbiol30