Table 1 |
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|
Axon outgrowth and guidance defects in unc-69 mutants |
||||||
| ALM defect (%) |
||||||
|
|
||||||
| Genotype |
B |
NR |
E |
FL |
n |
|
|
|
||||||
| Wild type |
0 |
0 |
0 |
12 |
113 |
|
| unc-69(e602) |
12 |
36 |
77 |
84 |
77 |
|
| unc-69(e587) |
15 |
45 |
85 |
89 |
80 |
|
| unc-69(e602) (m+z-) |
0 |
4.3 |
39 |
91 |
70 |
|
| unc-69(e602)/nDf40 (m+z-) |
0 |
7.2 |
20 |
72 |
69 |
|
| unc-69(e587) (m+z-) |
1.4 |
7.2 |
43 |
87 |
69 |
|
| unc-69(e587)/nDf40 (m+z-) |
0 |
8.3 |
20 |
82 |
60 |
|
| unc-69(e602)* |
19 |
48 |
62 |
95 |
113 |
|
| unc-69(e602); opEx [P mec -7 ::unc-69]* |
1 |
12 |
2 |
5 |
81 |
|
| unc-69(e602); opEx [P mec -7 ::unc-69]* |
5 |
9 |
6 |
10 |
79 |
|
| unc-69(e602); opEx [P mec -7 ::unc-69]* |
8 |
29 |
0 |
10 |
85 |
|
| AVM defect (%) |
||||||
|
|
||||||
| Genotype |
B |
NR |
E |
FL |
V |
n |
|
|
||||||
| Wild type |
0 |
0 |
1 |
8 |
0 |
106 |
| unc-69(e602) |
32 |
27 |
72 |
73 |
2.7 |
77 |
| unc-69(e587) |
64 |
70 |
86 |
87 |
0 |
80 |
| unc-69(e602) (m+z-) |
1.4 |
4.3 |
57 |
86 |
0 |
70 |
| unc-69(e602)/nDf40 (m+z-) |
0 |
0 |
56 |
80 |
0 |
69 |
| unc-69(e587) (m+z-) |
2.9 |
8.8 |
56 |
85 |
0 |
69 |
| unc-69(e587)/nDf40 (m+z-) |
0 |
3.6 |
76 |
95 |
0 |
60 |
| unc-69(e602)* |
46 |
67 |
93 |
100 |
ND |
113 |
| unc-69(e602); opEx [P mec -7 ::unc-69]* |
0 |
4 |
4 |
4 |
ND |
81 |
| unc-69(e602); opEx [P mec -7 ::unc-69]* |
8 |
12 |
12 |
23 |
ND |
79 |
| unc-69(e602); opEx [P mec -7 ::unc-69]* |
11 |
21 |
12 |
13 |
ND |
85 |
|
|
||||||
|
Neurite outgrowth and guidance defects of mechanosensory touch neurons in unc-69 mutants. The morphology of neurites of ALM (top) and AVM (bottom) neurons (as in the schematic in Figure 2) was scored in different unc-69 mutants, in unc-69/nDf40 heterozygotes, and in mosaic animals carrying a functional unc-69 transgene under the control of the mec-7 promoter, which directs expression in the six touch neurons. All worms scored had a Pmec-4::gfp transgene zdIs5 in the background to allow visualization of the neurite morphology. One ALM neurite was scored per animal. B, failure to form proper branch at the nerve ring; NR, failure of nerve ring branch to fully extend; E: failure to elongate past the branch point; FL, failure to extend fully; V, ventral guidance defect. (m+z-): homozygous mutant animals derived from heterozygous mothers. *These strains also carry a lin-15(n765) mutation in the background. All opEx transgenes also carry a wild-type copy of lin-15(+) as a coinjection marker. ND, not done. n, number of worms scored. |
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|
Su et al. Journal of Biology 2006 5:9 doi:10.1186/jbiol39 |
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