Table 1

Axon outgrowth and guidance defects in unc-69 mutants

ALM defect (%)


Genotype

B

NR

E

FL

n


Wild type

0

0

0

12

113

unc-69(e602)

12

36

77

84

77

unc-69(e587)

15

45

85

89

80

unc-69(e602) (m+z-)

0

4.3

39

91

70

unc-69(e602)/nDf40 (m+z-)

0

7.2

20

72

69

unc-69(e587) (m+z-)

1.4

7.2

43

87

69

unc-69(e587)/nDf40 (m+z-)

0

8.3

20

82

60

unc-69(e602)*

19

48

62

95

113

unc-69(e602); opEx [P mec -7 ::unc-69]*

1

12

2

5

81

unc-69(e602); opEx [P mec -7 ::unc-69]*

5

9

6

10

79

unc-69(e602); opEx [P mec -7 ::unc-69]*

8

29

0

10

85

AVM defect (%)


Genotype

B

NR

E

FL

V

n


Wild type

0

0

1

8

0

106

unc-69(e602)

32

27

72

73

2.7

77

unc-69(e587)

64

70

86

87

0

80

unc-69(e602) (m+z-)

1.4

4.3

57

86

0

70

unc-69(e602)/nDf40 (m+z-)

0

0

56

80

0

69

unc-69(e587) (m+z-)

2.9

8.8

56

85

0

69

unc-69(e587)/nDf40 (m+z-)

0

3.6

76

95

0

60

unc-69(e602)*

46

67

93

100

ND

113

unc-69(e602); opEx [P mec -7 ::unc-69]*

0

4

4

4

ND

81

unc-69(e602); opEx [P mec -7 ::unc-69]*

8

12

12

23

ND

79

unc-69(e602); opEx [P mec -7 ::unc-69]*

11

21

12

13

ND

85


Neurite outgrowth and guidance defects of mechanosensory touch neurons in unc-69 mutants. The morphology of neurites of ALM (top) and AVM (bottom) neurons (as in the schematic in Figure 2) was scored in different unc-69 mutants, in unc-69/nDf40 heterozygotes, and in mosaic animals carrying a functional unc-69 transgene under the control of the mec-7 promoter, which directs expression in the six touch neurons. All worms scored had a Pmec-4::gfp transgene zdIs5 in the background to allow visualization of the neurite morphology. One ALM neurite was scored per animal. B, failure to form proper branch at the nerve ring; NR, failure of nerve ring branch to fully extend; E: failure to elongate past the branch point; FL, failure to extend fully; V, ventral guidance defect. (m+z-): homozygous mutant animals derived from heterozygous mothers. *These strains also carry a lin-15(n765) mutation in the background. All opEx transgenes also carry a wild-type copy of lin-15(+) as a coinjection marker. ND, not done. n, number of worms scored.

Su et al. Journal of Biology 2006 5:9   doi:10.1186/jbiol39

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