Table 3 |
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|
Quantitative analysis of axon extension defects in unc-69(lf), unc-76(lf) and other mutants |
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|
Genotype |
2 AWCOFF (%) |
1 AWCON (%) |
2 AWCON (%) |
n |
|
|
||||
|
Wild type |
1 |
99 |
0 |
442 |
|
unc-69 |
||||
|
unc-69(ju69) |
1 |
99 |
0 |
190 |
|
unc-69(e602) |
34 |
66 |
0 |
119 |
|
unc-69(e587) |
30 |
70 |
0 |
194 |
|
unc-76 |
||||
|
unc-76(rh116) |
11 |
89 |
0 |
83 |
|
unc-76(n2457) |
6 |
94 |
0 |
102 |
|
unc-76(n2397) |
8 |
92 |
0 |
64 |
|
unc-76(ev424) |
10 |
90 |
0 |
68 |
|
unc-76(n2367) |
30 |
70 |
0 |
84 |
|
unc-76(n2399) |
25 |
75 |
0 |
67 |
|
unc-76(e911) |
47 |
53 |
0 |
101 |
|
unc-76(e911); lon-2(e678) |
31 |
69 |
0 |
91 |
|
unc-76(n2398) |
28 |
72 |
0 |
184 |
|
Double mutants |
||||
|
unc-69(e602); unc-76(n2457) |
35 |
65 |
0 |
106 |
|
unc-69(e602); unc-76(e911) |
48 |
52 |
0 |
118 |
|
unc-69(e587); unc-76(n2457) |
33 |
67 |
0 |
108 |
|
unc-69(e587); unc-76(e911) |
31 |
69 |
0 |
143 |
|
Other axonal guidance mutants |
||||
|
sax-3(ky123) |
64 |
33 |
3 |
112 |
|
lon-2(e678) unc-6(n102) |
38 |
62 |
0 |
65 |
|
vab-3(e648) |
54 |
40 |
6 |
68 |
|
unc-33(e204) |
7 |
73 |
20 |
135 |
|
unc-119(ed3) |
12 |
48 |
39 |
99 |
|
Other double mutants |
||||
|
unc-76(e911); sax-3(ky123) |
95 |
5 |
0 |
22 |
|
unc-76(e911); lon-2(e678) unc-6(n102) |
73 |
27 |
0 |
152 |
|
unc-76(e911); vab-3(e648) |
63 |
27 |
10 |
62 |
|
unc-33(e204); unc-76(e911) |
80 |
18 |
1 |
291 |
|
unc-119(ed3); unc-76(e911) |
65 |
32 |
3 |
167 |
|
|
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|
All animals scored had kyIs140 (Pstr-2::gfp) in the background, which turns on its expression in only one of the two AWC neurons (1 AWCON) in wild-type animals. In axon guidance mutants, Pstr-2::gfp expression is silenced in both AWCs (2 AWCOFF) owing to failure of axonal contact. All unc-69 and unc-76 alleles except unc-76(rh116) are arranged in order according to their physical position (5' to 3') in the open reading frame. n, number of animals scored. |
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|
Su et al. Journal of Biology 2006 5:9 doi:10.1186/jbiol39 |
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