Table 3

Quantitative analysis of axon extension defects in unc-69(lf), unc-76(lf) and other mutants

Genotype

2 AWCOFF (%)

1 AWCON (%)

2 AWCON (%)

n


Wild type

1

99

0

442

unc-69

unc-69(ju69)

1

99

0

190

unc-69(e602)

34

66

0

119

unc-69(e587)

30

70

0

194

unc-76

unc-76(rh116)

11

89

0

83

unc-76(n2457)

6

94

0

102

unc-76(n2397)

8

92

0

64

unc-76(ev424)

10

90

0

68

unc-76(n2367)

30

70

0

84

unc-76(n2399)

25

75

0

67

unc-76(e911)

47

53

0

101

unc-76(e911); lon-2(e678)

31

69

0

91

unc-76(n2398)

28

72

0

184

Double mutants

unc-69(e602); unc-76(n2457)

35

65

0

106

unc-69(e602); unc-76(e911)

48

52

0

118

unc-69(e587); unc-76(n2457)

33

67

0

108

unc-69(e587); unc-76(e911)

31

69

0

143

Other axonal guidance mutants

sax-3(ky123)

64

33

3

112

lon-2(e678) unc-6(n102)

38

62

0

65

vab-3(e648)

54

40

6

68

unc-33(e204)

7

73

20

135

unc-119(ed3)

12

48

39

99

Other double mutants

unc-76(e911); sax-3(ky123)

95

5

0

22

unc-76(e911); lon-2(e678) unc-6(n102)

73

27

0

152

unc-76(e911); vab-3(e648)

63

27

10

62

unc-33(e204); unc-76(e911)

80

18

1

291

unc-119(ed3); unc-76(e911)

65

32

3

167


All animals scored had kyIs140 (Pstr-2::gfp) in the background, which turns on its expression in only one of the two AWC neurons (1 AWCON) in wild-type animals. In axon guidance mutants, Pstr-2::gfp expression is silenced in both AWCs (2 AWCOFF) owing to failure of axonal contact. All unc-69 and unc-76 alleles except unc-76(rh116) are arranged in order according to their physical position (5' to 3') in the open reading frame. n, number of animals scored.

Su et al. Journal of Biology 2006 5:9   doi:10.1186/jbiol39

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