Open Access Highly Accessed Research article

High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry

Claude Lechene1*, Francois Hillion2, Greg McMahon1, Douglas Benson3, Alan M Kleinfeld4, J Patrick Kampf4, Daniel Distel5, Yvette Luyten5, Joseph Bonventre6, Dirk Hentschel6, Kwon Moo Park6, Susumu Ito7, Martin Schwartz8, Gilles Benichou9 and Georges Slodzian10

Author Affiliations

1 National Resource for Imaging Mass Spectrometry, Harvard Medical School and Department of Medicine, Brigham and Women's Hospital, Cambridge, MA 02139, USA

2 Cameca, 29 Quai des Gresillons, 92622 Gennevilliers Cedex, France

3 NSee Inc., 106 Greenhaven Lane, Cary, NC 27511, USA

4 Torrey Pines Institute for Molecular Studies, San Diego, CA 92121, USA

5 Ocean Genome Legacy Foundation, Ipswich, MA 01938, USA

6 Harvard Medical School and Renal Division, Brigham and Women's Hospital, Boston, MA 02115, USA

7 Harvard Medical School, Boston, MA 02115, USA

8 Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA

9 Harvard Medical School and Department of Surgery, Massachusetts General Hospital, Boston, MA 02114, USA

10 Universite Paris-Sud, Centre de Spectrométrie Nucléaire et de Spectrométrie de Masse, 91406 Orsay, France

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Journal of Biology 2006, 5:20  doi:10.1186/jbiol42

Published: 5 October 2006

Additional files

Additional data file 1:

An explanation of how the volume sputtered, the useful yield, and the detectability limit are calculated

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Additional data file 2:

An explanation of how the lateral resolution is calculated

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Additional data file 3:

Data reduction from labeled and control mouse cochlear sections

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Additional data file 4:

The relationship between the brightness of an image and the number of counts

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Additional data file 5:

A comparison of MIMS with autoradiography

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Additional data file 6:

An explanation of the shape of mass line peaks in MIMS

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Additional data file 7:

More details on Figure 2e

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