Research article
High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry
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* Corresponding author: Claude Lechene cpl@harvard.edu
1 National Resource for Imaging Mass Spectrometry, Harvard Medical School and Department of Medicine, Brigham and Women's Hospital, Cambridge, MA 02139, USA
2 Cameca, 29 Quai des Gresillons, 92622 Gennevilliers Cedex, France
3 NSee Inc., 106 Greenhaven Lane, Cary, NC 27511, USA
4 Torrey Pines Institute for Molecular Studies, San Diego, CA 92121, USA
5 Ocean Genome Legacy Foundation, Ipswich, MA 01938, USA
6 Harvard Medical School and Renal Division, Brigham and Women's Hospital, Boston, MA 02115, USA
7 Harvard Medical School, Boston, MA 02115, USA
8 Department of Microbiology, University of Virginia, Charlottesville, VA 22908, USA
9 Harvard Medical School and Department of Surgery, Massachusetts General Hospital, Boston, MA 02114, USA
10 Universite Paris-Sud, Centre de Spectrométrie Nucléaire et de Spectrométrie de Masse, 91406 Orsay, France
Journal of Biology 2006, 5:20 doi:10.1186/jbiol42
Published: 5 October 2006Additional files
Additional data file 1:
An explanation of how the volume sputtered, the useful yield, and the detectability limit are calculated
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Additional data file 2:
An explanation of how the lateral resolution is calculated
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Additional data file 3:
Data reduction from labeled and control mouse cochlear sections
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Additional data file 4:
The relationship between the brightness of an image and the number of counts
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Additional data file 5:
A comparison of MIMS with autoradiography
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Additional data file 6:
An explanation of the shape of mass line peaks in MIMS
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Additional data file 7:
More details on Figure 2e
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