Figure 7.

The bar-1 module regulates fat storage and/or metabolism. (a) The 'bar-1 module' of 21 genes was identified by virtue of the interconnectedness of coexpression, co-phenotype, genetic, and protein interactions within the superimposed network. Edges are colored according to the type of supporting evidence. Genes tested for interaction with bar-1 within the original SGI matrix are indicated (black dot). Visualization generated with Visant [86]. (b) Fat accumulation and/or storage disruption in the bar-1 module. Genes in the bar-1 module were targeted by RNAi in an N2 background. The resulting worms were stained with Nile Red and staining was quantified in order to compare values to N2 worms fed negative control RNAi (see Materials and methods). Fifteen of 20 genes show a reduction of Nile Red staining in an N2 background. Values have been normalized with N2 values for each experiment. Error bars represent standard error of the mean. (c,e) Dark-field micrographs of Nile Red staining (shows as bright patches) in N2 worms fed either (c) negative control mock-RNAi (∅ RNAi) or (e) RNAi that targets T20B12.7. (d,f) The corresponding differential interference contrast micrographs are shown below the dark-field micrographs. Scale bar, 50 μm.