Email updates

Keep up to date with the latest news and content from Journal of Biology and BioMed Central.

Highly Accessed Minireview

Making the jump: new insights into the mechanism of trans-translation

Jacek Wower1, Iwona K Wower1 and Christian Zwieb2*

Author Affiliations

1 Department of Animal Sciences, Auburn University, Auburn, AL 36849, USA.

2 Department of Molecular Biology, University of Texas Health Science Center at Tyler, 11937 US Highway 271, Tyler, TX 75708, USA.

For all author emails, please log on.

Journal of Biology 2008, 7:17  doi:10.1186/jbiol78


The electronic version of this article is the complete one and can be found online at: http://jbiol.com/content/7/5/17


Published:30 June 2008

© 2008 BioMed Central Ltd

Abstract

The transfer-messenger ribonucleoprotein (tmRNP), which is composed of RNA and a small protein, small protein B (SmpB), recycles ribosomes that are stalled on broken mRNAs lacking stop codons and tags the partially translated proteins for degradation. Although it is not yet understood how the ribosome gets from the 3' end of the truncated message onto the messenger portion of the tmRNA to add the tag, a recent study in BMC Biology has shed some light on this astonishing feat.

Discovery and properties of transfer messenger RNA

tmRNA was discovered in 1995 [1], when Simpson and coworkers overexpressed a mouse cytokine in Escherichia coli and found truncated cytokine peptides each tagged at the carboxyl termini with the same 11-amino acid residue extension AANDENYALAA. This tag sequence turned out to be encoded in a small stable RNA that had been identified many years earlier as a 10S RNA of unknown function [2]. The 10S RNA is now known as transfer messenger RNA (tmRNA). As its name implies, tmRNA has features of both transfer RNA and messenger RNA. One domain of the molecule, known as the transfer RNA-like domain (TLD), has an amino acid acceptor stem chargeable with alanine and a T arm with modified nucleotides, just as in tRNA (Figure 1). However, the D arm of the tRNA-like domain is degenerated, and there is no anticodon loop. A second domain, the mRNA-like domain (MLD), is located in a pseudoknot-rich region and contains a short open reading frame that encodes AANDENYALAA and is followed by a normal stop codon. It was quickly established that this peptide targets the truncated ribosomal product for degradation [3].

thumbnailFigure 1. Comparison of the structures of (a) tRNA, (b) mRNA and (c) tmRNA. (a, c) The 3' and 5' termini, the amino acid acceptor stem (AC) and the anticodon (A), D and T arms are indicated. (b, c) The Shine-Dalgarno sequence (SD), the start codon (s) and the stop codon (octagon), the locations of the tRNA-like (TLD) and mRNA-like domains (MLD) as well as pseudoknots (pk) 1 to 4, helix 5 (h5), and the +1 resume codon (r) are indicated. The thin arrows depict the pseudoknot connections.

These observations led to the proposal that the tmRNA occupies the empty A site of the stalled ribosome which then jumps or slides from the 3' end of the truncated message onto the MLD, at a triplet known as the resume codon (in E. coli this is a GCA triplet) from where translation continues normally until an in-frame tmRNA stop codon is encountered (Figure 2). This process is known as trans-translation [3]. In nature, bacteria use this seemingly complicated trick to proteolytically destroy proteins that are synthesized from damaged mRNA templates and, perhaps more importantly, to reactivate and recycle needed ribosomes [4]. In some bacteria, the gene for tmRNA (ssrA) is essential [5-7], but in other species trans-translation is important only to survive challenging environmental growth conditions, and this is probably the reason for the relatively late discovery of this fundamental capability of every bacterial cell.

thumbnailFigure 2. Steps in trans-translation. A ribosome remains stalled near the 3' end of broken mRNA, binds to alanine-charged tmRNA (orange), and switches from the broken message onto the open reading frame of the tmRNA allowing regular translation to resume. Upon reaching the tmRNA stop codon, the ribosome releases a hybrid protein with a degradation tag and joins the pool of active ribosomes.

The mechanism of trans-translation however is mysterious. Because the TLD of tmRNA has no anticodon, it is not clear how it can recognize and bind to the empty A site of a stalled ribosome (Figure 2). Moreover, the MLD has neither an AUG start codon nor the Shine-Dalgarno sequence whereby bacterial mRNA binds to a complementary region of the ribosomal RNA at the start of translation. How then is the resume triplet properly positioned? And what mechanism allows the ribosome to take off from the damaged mRNA template and land precisely on the tmRNA's resume codon? Astonishingly, the ribosome performs this feat when a peptide bond forms between the partially synthesized protein and the alanine-charged tmRNA, and while establishing the correct reading frame for continuing elongation. Miller and colleagues [8] have now carried out a systematic site-directed mutagenesis study in an attempt to establish the contribution of the nucleotide residues that precede the resume codon to the correct positioning of the MLD.

Identifying determinants of template switching

One problem in determining the critical elements of trans-translation in vivo has been that E. coli cells grow well without the ssrA gene, so mutations cannot be detected by their effects on growth. Furthermore, the tagged proteins produced by trans-translation are degraded, and therefore cannot be used to indicate whether it is occurring normally. Luckily, however, a wide variety of tag templates are tolerated, and, upon removal of the natural stop codons, large additions can be engineered onto the tmRNA and are then translated [9]. The group of Allen Buskirk has used an ingenious assay in which proper tagging of truncated kanamycin resistance (KanR) gene products on stalled ribosomes produces full-length KanR protein, so that E. coli survives on kanamycin plates only when the tmRNP is functional [10].

The nucleotides surrounding the resume codon have been the focus of several studies aimed at determining what enables the ribosome to switch templates (reviewed in [11]). The upstream region contains an adenosine-rich cluster of about seven residues adjacent to three nucleotides (the -1 triplet) immediately preceding the +1 guanosine. Downstream of the resume triplet, for unknown reasons, codons +2 to +4 prefer adenosine at the second position (Figure 3). On the basis of sequence comparisons and the idea that the -1 triplet (GUC, at positions 87–89 of E. coli tmRNA, Figure 3) should be in the A conformation for allowing tmRNA to participate in the ribosomal elongation cycle, it was proposed that the -1 triplet has a crucial role in template switching. Specifically, if the A conformation is required, 18 out of the 64 theoretically possible -1 triplets are prohibited, so they would yield tmRNAs that could not function in trans-translation [12].

thumbnailFigure 3. RNA structure logo [19] displaying the information content surrounding the tmRNA resume codon. The height of each symbol is proportional to its frequency in 486 representative sequences from an alignment of 730 tmRNAs [20]. Residues are numbered in reference to E. coli tmRNA [21]. The resume codon (+1), codons +2 to +4 and the -1 triplet are indicated.

The new systematic in vivo study from the Buskirk laboratory that has recently been published in BMC Biology [8] provides strong experimental evidence that the previously suspected -1 resume triplet has only a minor role in accommodating tmRNA on the ribosome. In this paper, Miller and colleagues [8] constructed mutant tmRNAs with all 64 possible permutations of the -1 triplet and determined their effect on survival in the kanamycin resistance assay. They found that eight of the 18 codons that were prohibited according to the -1 hypothesis [12] were in fact fully functional, and other mutant tmRNAs that were predicted by the -1 triplet rule to be functional were shown by experiment to be completely inactive. The results of this comprehensive study show that the proposed rule for the -1 triplet is invalid and suggest different nucleotides that are important for accommodation of tmRNA on the ribosome.

One alternative nucleotide is the highly conserved adenosine at position 86 of E. coli tmRNA (Figure 3), which was observed earlier to be important in trans-translation [13]. Indeed, by measuring survival in the kanamycin-resistance assay, the investigators confirmed that changing A86 to a pyrimidine yielded cells that were unable to trans-translate.

Because high-resolution structures of the ribosome-bound tmRNA at various stages of trans-translation are currently unavailable, it is unclear why the conserved A86 has such a prominent role. Although this adenosine residue may act independently to interact with the ribosome, the investigators suggest that the A86 interacts with a yet to be identified ligand that is primarily responsible for engaging the resume triplet and tmRNA in the attachment and synthesis of the tag peptide. They speculate that A86 might bind to the SmpB that is part of the transfer-messenger RNA ribonucleoprotein, or to ribosomal protein S1, two proteins that have been found by other investigators to be close to the decoding center of the ribosome-bound tmRNA at some stage of trans-translation [14-18]. Further studies at the atomic level will be required before the athletic potential of the ribosome is fully understood.

Acknowledgements

The authors were supported by grants GM58267 and GM49034 from the NIH. We dedicate this work to the late Twix.

References

  1. Tu GF, Reid GE, Zhang JG, Moritz RL, Simpson RJ: C-terminal extension of truncated recombinant proteins in Escherichia coli with a 10Sa RNA decapeptide.

    J Biol Chem 1995, 270:9322-9326. PubMed Abstract | Publisher Full Text OpenURL

  2. Ray BK, Apirion D: Characterization of 10S RNA: a new stable RNA molecule from Escherichia coli.

    Mol Gen Genet 1979, 174:25-32. PubMed Abstract | Publisher Full Text OpenURL

  3. Keiler KC, Waller PR, Sauer RT: Role of a peptide tagging system in degradation of proteins synthesized from damaged messenger RNA.

    Science 1996, 271:990-993. PubMed Abstract | Publisher Full Text OpenURL

  4. Karzai AW, Roche ED, Sauer RT: The SsrA-SmpB system for protein tagging, directed degradation and ribosome rescue.

    Nat Struct Biol 2000, 7:449-455. PubMed Abstract | Publisher Full Text OpenURL

  5. Huang C, Wolfgang MC, Withey J, Koomey M, Friedman DI: Charged tmRNA but not tmRNA-mediated proteolysis is essential for Neisseria gonorrhoeae viability.

    EMBO J 2000, 19:1098-1107. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  6. Hutchison CA, Peterson SN, Gill SR, Cline RT, White O, Fraser CM, Smith HO, Venter JC: Global transposon mutagenesis and a minimal Mycoplasma genome.

    Science 1999, 286:2165-2169. PubMed Abstract | Publisher Full Text OpenURL

  7. Watanabe T, Sugita M, Sugiura M: Identification of 10Sa RNA (tmRNA) homologues from the cyanobacterium Synechococcus sp. strain PCC6301 and related organisms.

    Biochim Biophys Acta 1998, 1396:97-104. PubMed Abstract | Publisher Full Text OpenURL

  8. Miller MR, Healy DW, Robison SG, Dewey JD, Buskirk AR: The role of upstream sequences in selecting the reading frame on tmRNA.

    BMC Biol 2008, 6:29. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text OpenURL

  9. Wower IK, Zwieb C, Wower J: Transfer-messenger RNA unfolds as it transits the ribosome.

    RNA 2005, 11:668-673. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  10. Tanner DR, Dewey JD, Miller MR, Buskirk AR: Genetic analysis of the structure and function of transfer messenger RNA pseudoknot 1.

    J Biol Chem 2006, 281:10561-10566. PubMed Abstract | Publisher Full Text OpenURL

  11. Moore SD, Sauer RT: The tmRNA system for translational surveillance and ribosome rescue.

    Annu Rev Biochem 2007, 76:101-124. PubMed Abstract | Publisher Full Text OpenURL

  12. Lim VI, Garber MB: Analysis of recognition of transfer-messenger RNA by the ribosomal decoding center.

    J Mol Biol 2005, 346:395-398. PubMed Abstract | Publisher Full Text OpenURL

  13. Williams KP, Martindale KA, Bartel DP: Resuming translation on tmRNA: a unique mode of determining a reading frame.

    EMBO J 1999, 18:5423-5433. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  14. Metzinger L, Hallier M, Felden B: Independent binding sites of small protein B onto transfer-messenger RNA during trans-translation.

    Nucleic Acids Res 2005, 33:2384-2394. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  15. Bessho Y, Shibata R, Sekine S, Murayama K, Higashijima K, Hori-Takemoto C, Shirouzu M, Kuramitsu S, Yokoyama S: Structural basis for functional mimicry of long-variable-arm tRNA by transfer-messenger RNA.

    Proc Natl Acad Sci USA 2007, 104:8293-8298. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  16. Valle M, Gillet R, Kaur S, Henne A, Ramakrishnan V, Frank J: Visualizing tmRNA entry into a stalled ribosome.

    Science 2003, 300:127-130. PubMed Abstract | Publisher Full Text OpenURL

  17. Wower J, Zwieb CW, Hoffman DW, Wower IK: SmpB: a protein that binds to double-stranded segments in tmRNA and tRNA.

    Biochemistry 2002, 41:8826-8836. PubMed Abstract | Publisher Full Text OpenURL

  18. Wower IK, Zwieb CW, Guven SA, Wower J: Binding and cross-linking of tmRNA to ribosomal protein S1, on and off the Escherichia coli ribosome.

    EMBO J 2000, 19:6612-6621. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  19. Gorodkin J, Heyer LJ, Brunak S, Stormo GD: Displaying the information contents of structural RNA alignments: the structure logos.

    Comput Appl Biosci 1997, 13:583-586. PubMed Abstract OpenURL

  20. Andersen ES, Rosenblad MA, Larsen N, Westergaard JC, Burks J, Wower IK, Wower J, Gorodkin J, Samuelsson T, Zwieb C: The tmRDB and SRPDB resources.

    Nucleic Acids Res 2006, 34:D163-D168. PubMed Abstract | Publisher Full Text | PubMed Central Full Text OpenURL

  21. Chauhan AK, Apirion D: The gene for a small stable RNA (10Sa RNA) of Escherichia coli.

    Mol Microbiol 1989, 3:1481-1485. PubMed Abstract | Publisher Full Text OpenURL