Transplanted GDAsCNTF express neurocan and NG2, but suppress host expression of these two CSPGs at 4 days post-injury/transplantation. (a) At 4 days after injury, control dorsal column injury margins express dense neurocan immunoreactivity (green) mainly associated with GFAP- processes. Note the absence of neurocan immunoreactivity in the injury center (to the left). (b,c) While neurocan immunoreactivity in host white matter was markedly lower and mainly associated with astrocyte cell bodies, many intra-injury GDAsCNTF within injury centers displayed neurocan immunoreativity. (d) NG2 immunoreactivity in control injuries is high in both injury centers and margins. (e,f) Although overall levels of NG2 immunoreactivity were reduced within injury centers and margins of GDACNTF-transplanted injury sites compared to untreated control injuries (compare (d) and (f)), levels of NG2 immunoreactivity were still higher than that previously observed for identical dorsal column injuries transplanted with GDAsBMP . Scale bars 200 μm.
Davies et al. Journal of Biology 2008 7:24 doi:10.1186/jbiol85