Table 1

Cellular dynamics in OX40-DTA mouse model [1]

Numbers (%)* (YFP+ (%))

Turnover

Death


CD4+

Naïve

-12 (8)

CFSE ↑, Ki67 ≈, BrdU ≈

ND§

Memory

0 (55)

Ki67 ↑, BrdU ≈

Treg

-40 (80)

Ki67 ↑, BrdU ↑

CD8+

Naïve

+4 (1)

CFSE ≈

ND

Memory

+133 (3)

BrdU ≈

ND

B cells

+53


*Percentage decrease or increase in cell population numbers compared with normal controls. Percentage of cell population marked for DTA-mediated deletion by the yellow fluorescent protein marker (YFP). Determined by loss of BrdU-labeled cells, which might also be influenced by dilution of label following proliferation. ND, not done; ≈, approximately equal to normal controls; ↑, increased compared with normal controls; §The increase in BrdU labeling was too low to be determined. The method by which cell turnover was assessed is indicated: BrdU, bromodeoxyuridine, is incorporated into the DNA of proliferating cells upon administration to mice; CFSE, carboxyfluorescein succinimidyl ester, is diluted out from adoptively transferred CFSE-labeled cells with each successive division; Ki67, a protein that is expressed in the nucleus of recently divided cells.

Vrisekoop et al. Journal of Biology 2009 8:91   doi:10.1186/jbiol198