Table 1 |
||||
|
Cellular dynamics in OX40-DTA mouse model [1] |
||||
|
Numbers (%)* (YFP+ (%)†) |
Turnover |
Death‡ |
||
|
|
||||
|
CD4+ |
Naïve |
-12 (8) |
CFSE ↑, Ki67 ≈, BrdU ≈ |
ND§ |
|
Memory |
0 (55) |
Ki67 ↑, BrdU ≈ |
↑ |
|
|
Treg |
-40 (80) |
Ki67 ↑, BrdU ↑ |
↑ |
|
|
CD8+ |
Naïve |
+4 (1) |
CFSE ≈ |
ND |
|
Memory |
+133 (3) |
BrdU ≈ |
ND |
|
|
B cells |
+53 |
|||
|
|
||||
|
*Percentage decrease or increase in cell population numbers compared with normal controls. †Percentage of cell population marked for DTA-mediated deletion by the yellow fluorescent protein marker (YFP). ‡Determined by loss of BrdU-labeled cells, which might also be influenced by dilution of label following proliferation. ND, not done; ≈, approximately equal to normal controls; ↑, increased compared with normal controls; §The increase in BrdU labeling was too low to be determined. The method by which cell turnover was assessed is indicated: BrdU, bromodeoxyuridine, is incorporated into the DNA of proliferating cells upon administration to mice; CFSE, carboxyfluorescein succinimidyl ester, is diluted out from adoptively transferred CFSE-labeled cells with each successive division; Ki67, a protein that is expressed in the nucleus of recently divided cells. |
||||
|
Vrisekoop et al. Journal of Biology 2009 8:91 doi:10.1186/jbiol198 |
||||