Resolution:
## Figure 7.
Influence of microbial exposure on immune homeostasis.
(a) Serum LBP levels (mean ± SEM, n = 6-9) in Ikbkg^{fl/Y}Vil-Cre (NEMO) and littermate control Ikbkg^{fl/Y}(WT) mice. The number above the bars is the P-value. (b) Total numbers (mean, n = 8-9) of B cells, T cells and macrophages (Mphi) in Ikbkg^{fl/Y }Vil-Cre (NEMO) and littermate control Ikbkg^{fl/Y }(WT) mice. P = 0.0007 for macrophages. (c) Flow cytometric example of CD11b^{+}F4/80^{+ }macrophage expansion in the spleen of NEMO mice, compared with WT mice. (d) CD4:CD8 ratio (± SEM) in the same mice. (e) Total numbers of naïve, memory and regulatory (reg) CD4^{+ }T cells and naïve and memory CD8^{+ }T cells from NEMO and control WT mice. (f) Serum LBP levels (mean ± SEM, n = 9-13) in Tnfrsf4^{Cre/+ }R26^{Dta/+ }(DTA) and control Tnfrsf4^{Cre/+ }R26^{+/+ }(WT) mice. (g) Total numbers (mean, n = 4-6) of B cells, T cells and macrophages (Mphi), (h) CD4:CD8 ratio and (i) total numbers of naïve, memory and regulatory (reg) CD4^{+ }T cells and naïve and memory CD8^{+ }T cells in Tnfrsf4^{Cre/+ }R26^{Dta/+}MyD88^{-/- }(DTA) and littermate control Tnfrsf4^{Cre/+ }R26^{+/+ }MyD88^{-/- }(-) mice. P = 0.035 for total cells; P = 0.015 for B cells; P = 0.0003 for regulatory CD4^{+ }T cells; P = 0.018 for total CD8^{+ }T cells; and P = 0.0003 for memory CD8^{+ }T cells. (j) CD62L and CD43 expression in gated memory CD44^{hi}CD8^{+ }T cells from the same mice. Numbers within bars denote the absolute number, ×10^{-7 }in (b, g), and ×10^{-6 }in (e, i), of each cell type.
Marques |