Apical polarity in three-dimensional culture systems: where to now?
Lawrence Berkeley National Laboratory, Division of Life Sciences, 1 Cyclotron Road, Berkeley, CA 94720, USA
Journal of Biology 2010, 9:2 doi:10.1186/jbiol213Published: 21 January 2010
Delineation of the mechanisms that establish and maintain the polarity of epithelial tissues is essential to understanding morphogenesis, tissue specificity and cancer. Three-dimensional culture assays provide a useful platform for dissecting these processes but, as discussed in a recent study in BMC Biology on the culture of mammary gland epithelial cells, multiple parameters that influence the model must be taken into account.