Robust and specific inhibition of microRNAs in Caenorhabditis elegans
Department of Molecular, Cellular and Development Biology, Yale University, New Haven, CT 06520, USA
Journal of Biology 2010, 9:20 doi:10.1186/jbiol230Published: 1 April 2010
MicroRNAs (miRNAs) are small non-coding RNAs that regulate the expression of numerous target genes. Yet, while hundreds of miRNAs have been identified, little is known about their functions. In a recent report published in Silence, Zheng and colleagues demonstrate a technique for robust and specific knockdown of miRNA expression in Caenorhabditis elegans using modified antisense oligonucleotides, which could be utilized as a powerful tool for the study of regulation and function of miRNAs in vivo.